WebDec 16, 2016 · One mL of bacteria was washed three times with 1X HBSS and resuspended in 1 mL of HBSS. Aliquots of 50 μL bacteria mixture were taken from each strain and put … WebMaterial i mètodes 70 - Solució B: 10 mM HEPES + 2 mM CaCl2 en HBSS— Es preparen 200 ml de cada solució concentrada 10x i 5x respectivament: - Solució A 10x: 4.76 g HEPES + 0.76 g EGTA - Solució B 5x: 2.38 g HEPES + 0.96 g …

Hank

WebHBSS 1X Hanks' Balanced Salt Solution is designed to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions. With: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate; … how to straighten a bent chrome bumper

Cytiva HyClone™ Hank

WebPBS (1X): 0.137 M NaCl, 0.05 M NaH 2 PO 4, pH 7.4 or Hank’s Balanced Salt Solution (HBSS; 1X) Flow Cytometry Staining Buffer (Catalog # FC001) or an equivalent solution containing BSA and sodium azide PI Staining … Web10-543Q HBSS (1X) without calcium or magnesium, with phenol red 1 L 10-547F HBSS (1X) without calcium, magnesium or phenol red 500 ml Hanks Balanced Salt Solution (HBSS) Title: HBSS_Product_Info_TS-04-315-3_10-09 Author: awinner Created Date: WebDec 16, 2016 · One mL of bacteria was washed three times with 1X HBSS and resuspended in 1 mL of HBSS. Aliquots of 50 μL bacteria mixture were taken from each strain and put in 1.5 mL Eppendorf Lo-bind tubes. Each tube containing bacteria was treated with 2 μL of 1 mg/mL BoNT/A complex for 4 h at 37 °C in a 90% humidity and 5% CO 2 incubator. readfield family market maine